Wohlschlaeger, a vice president and general counsel of Maytag Corp. when Ward was CEO there, submitted his resignation to the USOC's acting president, Bill Martin, effective immediately.
It was the eighth resignation of a high-ranking USOC official since the latest administrative turmoil began nearly three months ago.
Martin said Wohlschlaeger agreed to a severance package 'substantially less' than provided by his contract.
Ward also forfeited a potential $380,000 severance payment when he resigned at the weekend.
Commented Martin: "I want to commend Fred for placing the interests of the USOC ahead of his own and for his contributions to our organization as chief operating officer. This is a clear reflection of his character, professionalism and dedication to our athletes."
Ward hired Wohlschlaeger as chief of staff in January 2002, just after he was picked to be the USOC's fourth CEO in less than two years. Wohlschlaeger was promoted to COO last July, with oversight of the committee's Development, Finance, Human Resources, Information Technology, International Games, Sport Performance and Paralympics divisions.
Fred Wohlschlaeger has quit as chief operating officer of the US Olympic Committee, three days after his employer Lloyd Ward resigned as chief executive amid questions about ethics and management decisions.
