Rick Ferman will continue in the post until a successor is selected.
In what has been a series of internal moves, Nick Saviano, director of coaching education for the USA Tennis high performance programme has also quit.
Last week the USTA recruited Paul Roetert as its new managing director and Eliot Teltscher director of tennis operations for its high performance programme.
The chief operating officer and executive director of the United States Tennis Association has announced he is to quit.
